In Vitro Morphogenic Performance Of Ginger (Zingiber Officinale) As Influenced By Media Variations

ABSTRACT

Ginger (Zingiber officinale Rose) is a perennial herb that grows from underground rhizomes. The demand for fresh and dry ginger and its essential oil in the world market is high. Ginger is propagated vegetatively using underground rhizomes. Most farmers use planting materials saved from previous harvest. These materials could have been sold for cash. Contingent on this, many farmers are reluctant to use healthy succulent rhizomes for planting. These are rather sold thereby resulting in acute shortage of planting materials. Tissue culture technique can be applied to mass-produce seedlings for distribution to ginger farmers. This however, is not cost effective now due to lack of the necessary materials such as agar required for tissue culture. It is on the basis of these considerations that the present research was set up to develop a locally available and cheap protocol that is reproducible in ginger plant tissue culture work with the following objectives: To determine the best pre-initiation treatment for in-vitro ginger multiplication; To determine the effect of plant growth hormones – auxin and cytokinin on in-vitro propagation of ginger at the initiation stage using agar as a gelling agent; To compare agar gelled medium with cassava gelled medium in in-vitro propagation of ginger at the initiation stage. The research was carried out in the tissue culture research laboratory of the National Root Crop Research Institute (NRCRI), Umudike Station, Umuahia, Abia State. The explants were collected from ginger germplasm of the Institute. The result shows that Sodium Hypochlorite (NaOCl) as 30% bleach and agitating for 20 minutes effectively controlled the rate of contamination; however, at longer duration of agitation (30 minutes) the explants were badly damaged. At 4 weeks after initiation, 0.7mg/l NAA in combination with 0.25mg/l BAP gave the highest mean of 3.0 buds per plant (P<0.05). Explants cultured in cassava gelled medium at four weeks after initiation produced more buds than those cultured in agar gelled medium (P>0.05).

TABLE OF CONTENTS

PAGE……………………………………………………………………………………………………………..i CERTIFICATION………………………………………………………………………………………………………ii

DEDICATION…………………………………………………………………………………………………………..iii

ACKNOWLEDGEMENTS…………………………………………………………………………………………iv

TABLE OF CONTENTS……………………………………………………………………………………………..v

LIST OF TABLES……………………………………………………………………………………………………..vi

LIST OF FIGURES…………………………………………………………………………………………………..vii

ABSTRACT…………………………………………………………………………………………………………….viii

shortage of planting materials.

Tissue culture technique can be applied to mass-produce seedlings for distribution to ginger farmers. This however, is not cost effective now due to lack of the necessary materials required for tissue culture. Most of the materials for tissue culture are not locally available and are therefore procured at high amount. One of such materials is the gelling agent, agar. Agar represents one of the most expensive tissue culture media components. It is widely used for preparing solid and semi-solid plant tissue culture media because it withstands temperatures of about 100⁰C without being denatured (Scholten and Pierik, 1998). Therefore, establishment of plant micro propagation laboratories must be based on cost effectiveness. Gelatin that is cheaper when used at a concentration of 100g/1 is currently not widely used because it denatures at 25⁰C. Methocel (methyl cellulose) an alginate is also cheaper than Agar. However, Adaoha and Roscoe (1982) observed that there are two disadvantages associated with the use of alginates. The first is that solutions cannot be autoclaved without alginate breakdown during sterilization at high temperatures. The second disadvantage is that, alginate reacts with divalent metal cations (Be²⁺, Mg²⁺, Zn²⁺, Cd²⁺, Hg²⁺ and Pb²⁺) which may cause nutrient deprivation.

It is on the basis of these considerations that the present research was set up to develop a locally available and cheap protocol that is reproducible in ginger plant tissue culture work with the following objectives:

• To determine the best pre-initiation treatment for in-vitro ginger multiplication.
• To determine the effect of plant growth hormones – auxin and cytokinin on in-vitro propagation of ginger using agar as a gelling agent.
• To compare agar gelled medium with cassava gelled medium in in-vitro propagation of ginger at the initiation stage.