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Download this complete Project material titled; The Effects Of Temperature And Type Of Mouse Bedding On The Infectivity Of Human Schistosome Type Cercariae In Mice with abstract, chapters 1-5, references, and questionnaire. Preview Abstract or chapter one below

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ABSTRACT

Five batches of mice each batch consisting of five
replicates were exposed to cercariae at the rate of 200
cercariae per mouse at 5°C, 15OC, 25°c, 35°C and 45°C. It was
observed that cercariae maintained at 25°C had the highest
infectivity (15.2%) and those maintained at 45°C the lowest
(1.8%). Infectivity of cercariae at 5°C was zero. Except for
mice exposed to cercariae at 5°C, the Hb, g/lOOml and PCV%, of
mice infected at other temperatures differed significantly
(P<0.05) from those of the uninfected control. The tissue and
faecal egg count, and wormload of mice infected at 15, 35 and
45°c also differed significantly (P<0.05) from those of mice
infected at 25°C.
In another experiment, freshly emerged cercariae were
stored at constant temperature (5°C) for 3,6,12 and 24 hour
periods after which they were slowly warmed to 25 ± 1°C and
used to infect four batches of mice, the batches corresponding
with the four periods of storage. A fifth batch of mice was
infected with freshly emerged cercariae and served as the
infected control while a sixth batch served as an uninfected
control. The results showed an inverse relationship between
infectivity of cercariae and duration of storage. The
wormload, tissue and faecal egg counts of infected mice
differed significantly (P<0.05) from those of the infected
(vii)
control regardless of duration of storage of cercariae while
the Hb, g/lOOml and PCV% of infected mice except those exposed
to cercariae cold stored for 24 hours differed significantly
(P<0.05) from those of the uninfected control.
In yet another experiment one batch of five mice was
reared on sawdust bedding and another batch of five mice was
reared on shredded paper bedding for 7 days. It was found that
infectivity of cercariae to mice maintained on shredded paper
was higher than to mice on sawdust bedding. For all the
indices of infectivity investigated, there was a significant
difference (P<0.05) between shredded paper bred and sawdust
bred mice.

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